Chromosome Analysis Using Spectral Karyotyping (SKY) (pdf.

The clinical application of spectral karyotyping (SKY?) in the analysis of prenatally diagnosed extra structurally abnormal chromosomes (ESACs).

This article is published with open access at Springerlink.com Abstract Spectral karyotyping is a novel technique for chromosome analysis that has been developed based on the approach of the fluorescence in situ hybridization technique.

Spectral karyotyping (SKY) is a FISH-based method that labels each chromosome with a different color, allowing the identification of the chromosomal origin of all elements of the examined karyotype. From: Molecular Diagnostics, 2010.

Spectral karyotyping (SKY) is a novel cytogenetic technique, has been developed to unambiguously display and identify all 24 humans chromosomes at one time without a priori knowledge of any abnormalities involved.

Proceedings Paper Spectral karyotyping (SKY) analysis of heritable effects of radiation-induced malignant transformation. SKY analysis revealed multiple translocations and, combined with G-banding, allowed the definition of targets for positional cloning of tumor related genes.. (United States) George McNamara, Applied Spectral Imaging.

A comprehensive karyotypic study on human hepatocellular carcinoma by spectral karyotyping. Finally, a detailed chromosomal analysis using multicolor spectral karyotyping (SKY) revealed that there were multiple chromosomal alterations in the c-myc-overexpressing cells that contained either one or two unmutated p53 alleles.

A karyogram is the pictorial alignment of the 22 pairs of homologous autosomes from one metaphase cell, sequentially numbered from 1 to 22 (largest to smallest, one exception is that chromosome 21 is shorter than chromosome 22) by their unique band patterns, and the two sex chromosomes, XX (female) or XY (male).Alignment of the normal homologues is centered with the centromere so that the.

Spectral karyotyping is a novel technique for chromosome analysis that has been developed based on the approach of the fluorescence in situ hybridization technique. Spectral karyotyping makes it feasible to diagnose a variety of diseases, because of its technology in painting each of the 24 human chromosomes with different colors.

We used spectral karyotyping (SKY), to compare the individual chromosomal instability of two chromosome types: chromosomes that have a primary aberration and chromosomes without an aberration, in 13 primary childhood neural tumors and seven cell lines.

A technique disclosing most information about chromosome modifications is the technique of choice for the analysis of chromosome alterations. The newly developed method for microdissection of fluorescence-labeled chromosomes (FISH-MD) can improve upon this expectation in combination with 24-color spectral karyotyping (SKY).

Thus, chromosomes or chromosome sections can be visualized and identified, allowing for the analysis of chromosomal rearrangements. In the case of spectral karyotyping, image processing software assigns a pseudo color to each spectrally different combination, allowing the visualization of the individually colored chromosomes.